ISSN 1301-143X | E-ISSN 1309-1484
Original Article
Cystic Fibrosis-Modulated Phenotypic Changes of Pseudomonas aeruginosa May Be a Critical Determinant for Respiratory Infections and Unresponsiveness to Antimicrobial Agents
1 İskenderun Devlet Hastanesi, İnfeksiyon Hastalıkları ve Klinik Mikrobiyoloji Kliniği, Hatay, Türkiye  
2 Adnan Menderes Üniversitesi, Tıp Fakültesi, İnfeksiyon Hastalıkları ve Klinik Mikrobiyoloji Anabilim Dalı, Aydın, Türkiye  
3 Sağlık Bakanlığı, Türkiye İlaç ve Tıbbi Cihaz Kurumu, Ankara, Türkiye  
4 Adnan Menderes Üniversitesi, Sağlık Bilimleri Enstitüsü, Mikrobiyoloji Anabilim Dalı, Aydın, Türkiye  
Klimik Dergisi 2019; 32: 22-28
DOI: 10.5152/kd.2019.07
Key Words: Cystic fibrosis, biofilms, Pseudomonas aeruginosa.


Objective: Cystic fibrosis (CF) patients are exposed to respiratory system pathogens mostly forming biofilms, and therefore therapeutic interventions frequently fail. In this study, we aimed to investigate the resulting phenotypic changes in Pseudomonas aeruginosa due to interaction between bacteria and respiratory epithelium of CF patients.


Methods: In vitro investigation of some phenotypic characteristics of P. aeruginosa such as biofilm formation, adhesion, and its growth rate was made in normal and CF bronchial (BEAS-2B and IB3-1) and tracheal (1HAEo- and CFTE29o−) epithelium. P. aeruginosa PAO1, a green fluorescent protein expressing strain, was used in the experiments.


Results: Adhesion of P. aeruginosa to CF bronchial epithelium had decreased and biofilm formation had increased in CF bronchial epithelium compared to normal bronchial epithelium. Moreover, P. aeruginosa was found to be the cause of morphological changes in the CF bronchial epithelium.


Conclusions: Our findings support that the changes in respiratory epithelium with CF directly affect bacterial phenotypic characteristics in infected CF patients, and these phenotypic changes may be the major determinant of unresponsiveness to antimicrobial agents during treatment of infections. Klimik Dergisi 2019; 32(1): 22-8.


Cite this article as: Ersoy MÖ, Sakarya S, Günay N, Yılmaz Ö. [Cystic fibrosis-modulated phenotypic changes of Pseudomonas aeruginosa may be a critical determinant for respiratory infections and unresponsiveness to antimicrobial agents]. Klimik Derg. 2019; 32(1): 22-8. Turkish.

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