Early Release

Correction

Correction to: Evaluation of Biofilm Formation and Antibacterial Susceptibility in Klebsiella and Acinetobacter Strains Isolated from Hospital-Acquired Pneumonia

İlkay Nur Can and Others

Correction

Correction to: Management of Chronic Hepatitis B Infection: A Consensus Report of the Study Group for Viral Hepatitis of the Turkish Society of Clinical Microbiology and Infectious Diseases-2023 Update

Neşe Demirtürk and Others

Early Release / Consensus Report

Use of the BioFire® FilmArray® Pneumonia Plus Rapid Syndromic Multiplex PCR Assay in Pneumonia Patients – An Expert Opinion Report

Elif Demirören-Çayırcı and Others

Most Read

  1. Pneumococcal Vaccine as One of the Immunization Coverage Targets for Adulthood Vaccines: A Consensus Report of the Study Group for Adult Immunization of the Turkish Society of Clinical Microbiology and Infectious Diseases
  2. Does Hepatitis B Affect Erectile Dysfunction and Lower Urinary Tract Symptoms?
  3. HIV Infection in Turkey: How Close Are We to the Target?
  4. Evaluation of HBeAg-Negative Chronic Hepatitis B Patients Who Had Undergone Liver Biopsy in Accordance With the Guidelines
  5. When to Stop Antiviral Therapy in Chronic Hepatitis B
  6. Diagnosis, Treatment and Prevention of Diabetic Foot Wounds and Infections: Turkish Consensus Report
  7. Crimean-Congo Haemorrhagic Fever: Treatment and Use of Ribavirin

Volume 38, Issue 3

Original Article

Rotavirus Antigen Positivity in Pediatric Patients: Retrospective Evaluation Based on Clinical and Laboratory Parameters

Şinasi Karvar
Affiliations
  • Alanya Alaaddin Keykubat Üniversitesi Tıp Fakültesi, Tıbbi Mikrobiyoloji Anabilim Dalı, Antalya, Türkiye
https://orcid.org/0000-0003-3174-9674
,
Elif Okumuş
Affiliations
  • Sağlık Bilimleri Üniversitesi Van Eğitim ve Araştırma Hastanesi, Tıbbi Mikrobiyoloji Laboratuvarı, Van, Türkiye
https://orcid.ord/0000-0003-2191-6651
  • Child
  • Gastroenteritis
  • pediatrics
  • rotavirus
  • water-electrolyte imbalance

Abstract

Objective: Rotavirus is one of the leading viral agents of acute gastroenteritis, particularly in children under five years of age. This study aimed to analyze the association of rotavirus antigen positivity with age groups, seasonal distribution, hospital admission units, and laboratory parameters to contribute to diagnostic processes.

Methods: This retrospective cross-sectional study included 23 568 children aged 0–18 years who underwent rotavirus antigen testing at Van Training and Research Hospital between January 1, 2022, and December 31, 2024. Demographic (age, sex) and clinical data were obtained retrospectively from the hospital information system (Karmed). Rotavirus antigen detection was performed on stool samples using the lateral flow (immunochromatographic) rapid test method.

Results: Rotavirus antigen positivity was detected in 19.3% of the patients. The highest positivity rate was observed in the 1–4 year age group (24.9%), while the lowest was in the 12–17 year age group (4.5%) (p<0.0001). Positivity rates increased significantly during the autumn and winter months. Laboratory analysis revealed significantly lower leukocyte and lymphocyte counts, as well as potassium levels, in the positive group, whereas the percentage of neutrophils was significantly higher (p<0.0001). No patients showed evidence of severe fluid–electrolyte loss. Receiver operating characteristic (ROC) analysis revealed the highest area under the curve (AUC) for urea (0.599); in multivariate models, the maximum AUC was 0.648, indicating limited diagnostic power of the parameters.

Conclusion: Rotavirus infections are most common in early childhood and during the winter months, generally presenting with a mild clinical course. Although statistically significant differences were found in laboratory parameters, they were not diagnostic in isolation and should be interpreted together with clinical findings. Our findings suggest that rotavirus test requests should be planned more effectively based on patient age, seasonal features, and symptomatic evaluation.

Show References

References

  1. Myat TW, Thu HM, Tate JE, et al. Rotavirus infection among children under five years of age hospitalized with acute gastroenteritis in Myanmar during 2018–2020 –multicentre surveillance before rotavirus vaccine introduction. Vaccine. 2021;39(47):6907-12. [CrossRef]
  2. Süzük S, Avciküçük H, Kavak M. [Frequency of rotavirus infection in children with acute gastroenteritis in Kırıkkale Yüksek İhtisas Hospital]. Turk Hij Den Biyol Derg. 2015;72(1):11-16. Turkish. [CrossRef] 
  3. Cohen AL, Platts-Mills JA, Nakamura T, et al. Aetiology and incidence of diarrhoea requiring hospitalisation in children under 5 years of age in 28 low-income and middle-income countries: Findings from the Global Pediatric Diarrhea Surveillance Network. BMJ Glob Health. 2022;7(9):e009548. [CrossRef]
  4. Crawford SE, Ramani S, Tate JE, et al. Rotavirus infection. Nat Rev Dis Primers. 2017;3:17083. [CrossRef] 
  5. Payne S. Family Reoviridae. In: Bamford DH, Zuckerman M, editors. Viruses. 1st ed. Cambridge (MA): Academic Press; 2017. p. 219-26
  6. Ozdemir S, Delialioğlu N, Emekdaş G. [Investigation of rotavirus, adenovirus and astrovirus frequencies in children with acute gastroenteritis and evaluation of epidemiological features]. Mikrobiyol Bul. 2010;44(4):571-8. Turkish. 
  7. Yesiloglu C, Kurt A, Akgun-Karapinar DB, Sahin-Aydin HA, Teksoy N, Ongen B. Rotavirus positivity in children and adolescents: Evaluation of ten-year results. Clin Lab. 2025;71(2). [CrossRef] 
  8. Gülbudak H, Kurnaz N, Tezcan-Ülger S, et al. [The investigation of rotavirus and adenovirus frequency among patients with acute gastroenteritis]. Turk Hij Den Biyol Derg. 2020;77(2):185-94. Turkish. [CrossRef]
  9. Pawłuszkiewicz K, Ryglowski PJ, Idzik N, et al. Rotavirus infections: pathophysiology, symptoms, and vaccination. Pathogens. 2025;14(5):480. [CrossRef]
  10. Zhang C, Li G, Zhang H, Zhang H, Fei Y. Decreased lymphocyte to monocyte ratio and increased neutrophil to lymphocyte ratio observed in rotavirus-positive acute gastroenteritis in children: A retrospective study. Ann Clin Lab Sci. 2020;50(4):450-6. 
  11. Avcioglu F, Behcet M. Investigation of rotavirus and enteric adenovirus antigens in children between 0–14 years old. Med Sci. 2020;9(3):9225. [CrossRef] 
  12. Çimen B, Aktaş O. Distribution of bacterial, viral and parasitic gastroenteritis agents in children under 18 years of age in Erzurum, Turkey, 2010-2020. Germs. 2022;12(4):444-51. [CrossRef] 
  13. Aytaç Ö, Şenol FF, Öner P, et al. [Rotavirus and adenovirus frequency in acute gastroenteric diseases]. Turk Hij Den Biyol Derg. 2020;77(2):179-84. Turkish. [CrossRef] 
  14. Aydın Z, Almış H, Cilasun C, Bucak İ, Turgut M. Evaluation of children with rotavirus positive and rotavirus negative diarrhea. J Child. 2023;23(2):113-18. [CrossRef] 
  15. Galani IE, Andreakos E. Neutrophils in viral infections: Current concepts and caveats. J Leukoc Biol. 2015;98(4):557-64. [CrossRef] 
  16. Leung AK, Kellner JD, Davies HD. Rotavirus gastroenteritis. Adv Ther. 2005;22(5):476-87. [CrossRef]
  17. Ramig RF. Systemic rotavirus infection. Expert Rev Anti Infect Ther. 2007;5(4):591-612. [CrossRef]
  18. Gubbari KTN. Electrolyte disturbance in rotaviral diarrhea and other acute diarrheal diseases in children under 5 years. Int J Contemp Pediatr. 2019;6(5):2077-80.
  19. Bose T, Borrow R, Arkwright PD. Impact of rotavirus vaccination on diarrheal disease burden of children in South America. Expert Rev Vaccines. 2024;23(1):606-18. [CrossRef]
  20. Diallo AO, Wikswo ME, Sulemana I, et al. Rotavirus vaccine effectiveness against severe acute gastroenteritis: 2009–2022. Pediatrics. 2024;154(4):e2024066879. [CrossRef]
  21. Lv YJ, Hu QL, Huang R, Zhang L, Wu LF, Fu S. The Diagnostic and therapeutic value of the detection of serum amyloid A and C-reactive protein in infants with rotavirus diarrhea. Int J Gen Med. 2021;14:3611-17. [CrossRef]
  • 162-7
  • Şinasi Karvar
  • [email protected]
  • 10.36519/kd.2025.5331
  • July 4, 2025
  • August 29, 2025
  • September 27, 2025

  • Karvar Ş, Okumuş E. [Rotavirus antigen positivity in pediatric patients: Retrospective evaluation based on clinical and laboratory parameters]. Klimik Derg. 2025;38(3):162-7. Turkish.
  • 300
  • 98
Download as PDF
  • This work is licensed under a Creative Commons Attribution-Non Commercial 4.0 International License. CC BY-NC
Volume 38, Issue 3 Volume 38, Issue 2 Volume 38, Issue 1 Volume 37, Issue 4 Volume 37, Issue 3 Volume 37, Issue 2 Volume 37, Issue 1 Volume 36, Issue 4 Volume 36, Supplement 1 Volume 36, Issue 3 Volume 36, Issue 2 Volume 36, Issue 1 Volume 35, Issue 4 Volume 35, Issue 3 Volume 35, Issue 2 Volume 35, Issue 1 Volume 34, Issue 3 Volume 34, Issue 2 Volume 34, Issue 1 Volume 33, Issue 3 Volume 33, Issue 2 Volume 33, Issue 1 Volume 32, Issue 3 Volume 32, Supplement 1 Volume 32, Supplement 2 Volume 32, Issue 2 Volume 32, Issue 1 Volume 31, Issue 3 Volume 31, Issue 2 Volume 31, Supplement 1 Volume 31, Issue 1 Volume 30, Issue 3 Volume 30, Issue 2 Volume 30, Supplement 1 Volume 30, Issue 1 Volume 29, Issue 3 Volume 29, Issue 2 Volume 29, Issue 1 Volume 28, Supplement 1 Volume 28, Issue 3 Volume 28, Issue 2 Volume 28, Issue 1 Volume 27, Supplement 1 Volume 27, Issue 3 Volume 27, Issue 2 Volume 27, Issue 1 Volume 26, Issue 3 Volume 26, Supplement 1 Volume 26, Issue 2 Volume 26, Issue 1 Volume 25, Issue 3 Volume 25, Issue 2 Volume 25, Issue 1 Volume 24, Issue 3 Volume 24, Issue 2 Volume 24, Issue 1 Volume 23, Issue 3 Volume 23, Issue 2 Volume 23, Issue 1 Volume 22, Issue 3 Volume 22, Issue 2 Volume 22, Issue 1 Volume 21, Issue 3 Volume 21, Supplement 2 Volume 21, Supplement 1 Volume 21, Issue 2 Volume 21, Issue 1 Volume 20, Issue 3 Volume 20, Supplement 2 Volume 20, Issue 2 Volume 20, Issue 1 Volume 20, Supplement 1 Volume 19, Issue 3 Volume 19, Issue 2 Volume 19, Issue 1 Volume 18, Issue 3 Volume 18, Supplement 1 Volume 18, Issue 2 Volume 18, Issue 1 Volume 17, Issue 3 Volume 17, Issue 2 Volume 17, Issue 1 Volume 16, Issue 3 Volume 16, Issue 2 Volume 16, Issue 1 Volume 1, Supplement 1 Volume 15, Issue 3 Volume 15, Issue 2 Volume 15, Issue 1 Volume 14, Issue 3 Volume 14, Issue 2 Volume 14, Issue 1 Volume 13, Issue 3 Volume 13, Issue 2 Volume 13, Supplement 1 Volume 13, Issue 1 Volume 12, Issue 3 Volume 12, Issue 2 Volume 12, Issue 1 Volume 11, Issue 3 Volume 11, Issue 2 Volume 11, Supplement 1 Volume 11, Issue 1 Volume 10, Issue 3 Volume 10, Issue 2 Volume 10, Issue 1 Volume 9, Issue 3 Volume 9, Issue 2 Volume 9, Issue 1 Volume 8, Issue 3 Volume 8, Issue 2 Volume 8, Issue 1 Volume 6, Issue 3 Volume 7, Issue 1 Volume 7, Issue 2 Volume 7, Issue 3 Volume 4, Issue 3 Volume 5, Issue 1 Volume 5, Issue 2 Volume 5, Issue 3 Volume 6, Issue 1 Volume 6, Issue 2 Volume 3, Issue 1 Volume 3, Issue 2 Volume 3, Issue 3 Volume 4, Issue 1 Volume 4, Issue 2 Volume 1, Issue 2 Volume 2, Issue 1 Volume 2, Issue 2 Volume 2, Issue 3 Volume 1, Issue 1