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Volume 38, Issue 3

Original Article

Investigation of Efflux Pump and Quorum Sensing Genes in Acinetobacter baumannii Isolates

Ebru Karadağ
Affiliations
  • Selçuk Üniversitesi Tıp Fakültesi, Tıbbi Mikrobiyoloji Anabilim Dalı, Konya, Türkiye
https://orcid.org/0000-0001-8004-1029
,
Masma Shahbazova
Affiliations
  • Selçuk Üniversitesi Tıp Fakültesi, Tıbbi Mikrobiyoloji Anabilim Dalı, Konya, Türkiye
https://orcid.org/0000-0002-3414-725X
,
Nurullah Çiftçi
Affiliations
  • Kafkas Üniversitesi Tıp Fakültesi, Tıbbi Mikrobiyoloji Anabilim Dalı, Kars, Türkiye
https://orcid.org/0000-0002-8934-0575
,
Uğur Arslan
Affiliations
  • Selçuk Üniversitesi Tıp Fakültesi, Tıbbi Mikrobiyoloji Anabilim Dalı, Konya, Türkiye
https://orcid.org/0000-0001-6974-9173
  • A. baumannii
  • antibiotic resistance
  • efflux pump
  • quorum sensing gene

Abstract

Objective: Acinetobacter baumannii can cause serious hospital infections, particularly in intensive care units (ICUs) and in immunocompromised patients. Prolonged ICU stay, antibiotic use, mechanical ventilation, intravenous catheters, urinary catheters, endotracheal tubes, tracheostomy, and a high density of infected or colonized patients in the unit are the main risk factors. Acinetobacter baumannii strains are clinically important due to their ability to develop high levels of resistance to antibiotics and to survive for long periods in hospital environments. This study aimed to investigate the efflux pump and quorum sensing genes in A. baumannii strains isolated from blood cultures sent to our laboratory.

Methods: Fifty-three multidrug-resistant A. baumannii strains isolated from blood cultures submitted to our hospital’s Medical Microbiology Laboratory were included. Species identification and minimum inhibitory concentration (MIC) values were determined using the VITEK 2 automated system (bioMérieux, France). Efflux pump genes (adeABC) and quorum sensing genes (luxI, luxR) were investigated by polymerase chain reaction (PCR). After PCR, all samples were evaluated by agarose gel electrophoresis to detect specific DNA bands.

Results: In this study, the bap gene was the least frequently detected gene (1.9%), while the efflux pump gene adeA was found in all isolates (100%). The other efflux pump genes were detected at the following rates: adeB 96.2%, adeC 45.3%, adeI 86.8%, adeJ 67.9%, adeK 98.1%, and adeR 98.1%. Among the quorum sensing genes, luxI was detected in 41.5% and luxR in 47.2% of the isolates. High levels of resistance were observed against carbapenems and quinolones, while no resistance was detected against colistin.

Conclusion: This study contributes to a better understanding of the epidemiology and pathogenesis of A. baumannii infections. Similar studies will help identify the genes involved in resistance mechanisms of multidrug-resistant A. baumannii strains and support the development of new treatment strategies and policies. Furthermore, identifying resistance factors in hospital-based studies will help select the most effective treatment methods and contribute positively to both patient health and the national economy.

Show References

References

  1. Blanchard C, Barnett P, Perlmutter J, Dunman PM. Identification of Acinetobacter baumannii serum-associated antibiotic efflux pump inhibitors. Antimicrob Agents Chemother. 2014;58(11):6360-70. [CrossRef]
  2. Almasaudi SB. Acinetobacter spp. as nosocomial pathogens: Epidemiology and resistance features. Saudi J Biol Sci. 2018;25(3):586-96. [CrossRef]
  3. Peleg AY, Seifert H, Paterson DL. Acinetobacter baumannii: emergence of a successful pathogen. Clin Microbiol Rev. 2008;21(3):538-82. [CrossRef]
  4. Blanco N, Harris AD, Rock C, et al; the CDC Epicenters Program. Risk factors and outcomes associated with multidrug-resistant Acinetobacter baumannii upon intensive care unit admission. Antimicrob Agents Chemother. 2017;62(1):e01631-17. [CrossRef] 
  5. Magiorakos AP, Srinivasan A, Carey RB, et al. Multidrug-resistant, extensively drug-resistant and pandrug-resistant bacteria: an international expert proposal for interim standard definitions for acquired resistance. Clin Microbiol Infect. 2012;18(3):268-81. [CrossRef] 
  6. Bitrian M, Solari CM, González RH, Nudel CB. Identification of virulence markers in clinically relevant strains of Acinetobacter genospecies. Int Microbiol. 2012;15(2):79-88. 
  7. Marchand I, Damier-Piolle L, Courvalin P, Lambert T. Expression of the RND-type efflux pump AdeABC in Acinetobacter baumannii is regulated by the AdeRS two-component system. Antimicrob Agents Chemother. 2004;48(9):3298-304. [CrossRef]
  8. Manchanda V, Sanchaita S, Singh N. Multidrug resistant Acinetobacter. J Glob Infect Dis. 2010;2(3):291-304. [CrossRef]
  9. GenBank. Acinetobacter baumannii biofilm-associated protein (bap) gene, partial cds [Internet]. Bethesda (MD): National Center for Biotechnology Information, U.S. National Library of Medicine; 2015. [erişim 25 Haziran 2025]. https://www.ncbi.nlm.nih.gov/nuccore/KR080550.1
  10. Lee CR, Lee JH, Park M, et al. Biology of Acinetobacter baumannii: pathogenesis, antibiotic resistance mechanisms, and prospective treatment options. Front Cell Infect Microbiol. 2017;7:55. [CrossRef] 
  11. Yang CH, Su PW, Moi SH, Chuang LY. Biofilm formation in Acinetobacter Baumannii: genotype-phenotype correlation. Molecules. 2019;24(10):1849. [CrossRef]
  12. Qi L, Li H, Zhang C, et al. Relationship between antibiotic resistance, biofilm formation, and biofilm-specific resistance in Acinetobacter baumannii. Front Microbiol. 2016;7:483. [CrossRef] 
  13. Modarresi F, Azizi O, Shakibaie MR, Motamedifar M, Valibeigi B, Mansouri S. Effect of iron on expression of efflux pump (adeABC) and quorum sensing (luxI, luxR) genes in clinical isolates of Acinetobacter baumannii. APMIS. 2015;123(11):959-68. [CrossRef]
  14. Goh HM, Beatson SA, Totsika M, et al. Molecular analysis of the Acinetobacter baumannii biofilm-associated protein. Appl Environ Microbiol. 2013;79(21):6535-43. [CrossRef]
  15. Sharma M, Sapkota J, Jha B, Mishra B, Bhatt CP. Biofilm formation and extended-spectrum beta-lactamase producer among Acinetobacter species isolated in a tertiary care hospital: a descriptive cross-sectional study. JNMA J Nepal Med Assoc. 2019;57(220):424-8. [CrossRef]
  16. Ranjbar R, Zayeri S, Afshar D. High frequency of adeA, adeB and adeC genes among Acinetobacter baumannii isolates. Iran J Public Health. 2020;49(8):1539-45. [CrossRef]
  17. Yoon EJ, Balloy V, Fiette L, Chignard M, Courvalin P, Grillot-Courvalin C. Contribution of the ade resistance-nodulation-cell division-type efflux pumps to fitness and pathogenesis of Acinetobacter baumannii. mBio. 2016;7(3):e00697-16. [CrossRef]
  18. Zarrilli R, Pournaras S, Giannouli M, Tsakris A. Global evolution of multidrug-resistant Acinetobacter baumannii clonal lineages. Int J Antimicrob Agents. 2013;41(1):11-9. [CrossRef]
  19. Wieczorek P, Sacha P, Czaban S, et al. Distribution of adeABC efflux system genes in genotypically diverse strains of clinical Acinetobacter baumannii. Diagn Microbiol Infect Dis. 2013;77(2):106-9. [CrossRef]
  • 168-72
  • Nurullah Çiftçi
  • [email protected]
  • 10.36519/kd.2025.5327
  • June 29, 2025
  • August 29, 2025
  • September 27, 2025

  • Karadağ E, Shahbazova M, Çiftçi N, Arslan U. [Investigation of efflux pump and quorum sensing genes in Acinetobacter baumannii isolates]. Klimik Derg. 2025;38(3):168-72. Turkish. 
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