Most Read

  1. Pneumococcal Vaccine as One of the Immunization Coverage Targets for Adulthood Vaccines: A Consensus Report of the Study Group for Adult Immunization of the Turkish Society of Clinical Microbiology and Infectious Diseases
  2. HIV Infection in Turkey: How Close Are We to the Target?
  3. Evaluation of HBeAg-Negative Chronic Hepatitis B Patients Who Had Undergone Liver Biopsy in Accordance With the Guidelines
  4. Does Hepatitis B Affect Erectile Dysfunction and Lower Urinary Tract Symptoms?
  5. Diagnosis, Treatment and Prevention of Diabetic Foot Wounds and Infections: Turkish Consensus Report
  6. When to Stop Antiviral Therapy in Chronic Hepatitis B
  7. Mycoplasma hominis and Ureaplasma urealyticum: Their Role in Urogenital System Infections and Retrospective Evaluation of Antibiotic Resistance Rates
  8. Crimean-Congo Haemorrhagic Fever: Treatment and Use of Ribavirin
  9. Modern Diagnostic Methods Used in Bacteriology: Rapid and Effective
  10. A Case of Generalized Tetanus With a Short Incubation Period
  11. Does Anti-HIV Positivity in Pregnancy Suggest the Requirement of HIV Prophylaxis for Newborn?
  12. Analysis of HIV/AIDS-Related Stigma and Discrimination in Turkey: Results of the People Living With HIV Stigma Index
  13. Potato Dextrose Agar With Rose-Bengal and Chloramphenicol: A New Culture Medium to Isolate Pathogenic Exophiala dermatitidis From the Environment

Volume 36, Issue 4

Original Article

Macrolide-Lincosamide-Streptogramin B (MLSB) Resistance Phenotypes and Mupirocin/Fusidic Acid Activity in Staphylococcus aureus Isolates from Clinical Samples

Çiğdem Arabacı
Affiliations
  • İstanbul Prof. Dr. Cemil Taşçıoğlu Şehir Hastanesi, Tıbbi Mikrobiyoloji Laboratuvarı, İstanbul, Türkiye
https://orcid.org/0000-0003-0050-3225
,
Berrin Uzun
Affiliations
  • İzmir Atatürk Eğitim Araştırma Hastanesi, Tıbbi Mikrobiyoloji Laboratuvarı, İzmir, Türkiye
https://orcid.org/0000-0001-9115-5910
,
Kenan Ak
Affiliations
  • İstanbul Prof. Dr. Cemil Taşçıoğlu Şehir Hastanesi, Tıbbi Mikrobiyoloji Laboratuvarı, İstanbul, Türkiye
https://orcid.org/0000-0001-5863-3685
  • D-test
  • fusidic acid sensitivity
  • lincosamides
  • mupirocin sensitivity
  • resistance to macrolides
  • Staphylococcus aureus
  • Streptogramin B (MLSB)

Abstract

Objective: This study aimed to determine the prevalence and phenotypes of macrolide, lincosamide, and streptogramın B (MLSB) resistance in Staphylococcus aureus strains isolated from clinical samples and to assess the efficacy of mupirocin and fusidic acid against these isolates.

Methods: Between January 2021 and December 2022, S. aureus strains isolated from clinical samples were identified by VITEK® MS (bioMérieux, France), and their antibiotic susceptibilities were determined using VITEK®2 (bioMérieux, France). The disk approximation method was used to determine the MLSB resistance phenotypes using erythromycin (15 μg) and clindamycin (2 μg). For fusidic acid, a MIC of ≤1 mg/L was considered susceptible, and a MIC of >1 mg/L was considered resistant. The European Committee on Antimicrobial Susceptibility Testing (EUCAST) 2020 guidelines were followed, with a MIC of ≤1 mg/L considered sensitive, a MIC of 2-256 mg/L indicating low-level resistance, and a MIC of >256 mg/L indicating high-level resistance for mupirocin.

Results: The MLSB resistance phenotypes of the strains were as follows: 8.9% inducible (iMLSB), 4.6% chromosomal (cMLSB), 12.8% MS type, 0.8% L type, and 72.92% erythromycin and clindamycin sensitive were detected. Mupirocin resistance was detected 7.2% in 250 S. aureus strains, 14.3% in MRSA, and 2.1% in MSSA. Fusidic acid resistance was seen at 10.7% in 711 S. aureus strains, 20.1% in MRSA, and 5.5% in MSSA.

Conclusion: When erythromycin resistance is detected in MRSA isolates, it is essential to determine macrolide resistance phenotypes with a simple and easy method such as the D-test and investigate clindamycin resistance for choosing appropriate antibiotics. Mupirocin resistance in S. aureus strains was higher in MRSA isolates than in MSSA isolates. It is noteworthy that fusidic acid resistance is frequently seen in both MRSA and MSSA isolates that are susceptible to erythromycin and clindamycin. In infections where fusidic acid is commonly used, it is necessary to monitor antibiotic susceptibilities closely.

  • 262-7
  • Çiğdem Arabacı
  • [email protected]
  • 10.36519/kd.2023.4705
  • July 31, 2023
  • October 24, 2023
  • December 25, 2023

  • Arabacı Ç, Uzun B, Ak K. [Macrolide-lincosamide-streptogramin B (MLSB) resistance phenotypes and mupirocin/fusidic acid activity in Staphylococcus aureus isolates from clinical samples]. Klimik Derg. 2023;36(4):262-7. Turkish.
  • 237
  • 59
Download as PDF
  • This work is licensed under a Creative Commons Attribution-Non Commercial 4.0 International License. CC BY-NC
Volume 37, Issue 1 Volume 36, Issue 4 Volume 36, Supplement 1 Volume 36, Issue 3 Volume 36, Issue 2 Volume 36, Issue 1 Volume 35, Issue 4 Volume 35, Issue 3 Volume 35, Issue 2 Volume 35, Issue 1 Volume 34, Issue 3 Volume 34, Issue 2 Volume 34, Issue 1 Volume 33, Issue 3 Volume 33, Issue 2 Volume 33, Issue 1 Volume 32, Issue 3 Volume 32, Supplement 1 Volume 32, Supplement 2 Volume 32, Issue 2 Volume 32, Issue 1 Volume 31, Issue 3 Volume 31, Issue 2 Volume 31, Supplement 1 Volume 31, Issue 1 Volume 30, Issue 3 Volume 30, Issue 2 Volume 30, Supplement 1 Volume 30, Issue 1 Volume 29, Issue 3 Volume 29, Issue 2 Volume 29, Issue 1 Volume 28, Supplement 1 Volume 28, Issue 3 Volume 28, Issue 2 Volume 28, Issue 1 Volume 27, Supplement 1 Volume 27, Issue 3 Volume 27, Issue 2 Volume 27, Issue 1 Volume 26, Issue 3 Volume 26, Supplement 1 Volume 26, Issue 2 Volume 26, Issue 1 Volume 25, Issue 3 Volume 25, Issue 2 Volume 25, Issue 1 Volume 24, Issue 3 Volume 24, Issue 2 Volume 24, Issue 1 Volume 23, Issue 3 Volume 23, Issue 2 Volume 23, Issue 1 Volume 22, Issue 3 Volume 22, Issue 2 Volume 22, Issue 1 Volume 21, Issue 3 Volume 21, Supplement 2 Volume 21, Supplement 1 Volume 21, Issue 2 Volume 21, Issue 1 Volume 20, Issue 3 Volume 20, Supplement 2 Volume 20, Issue 2 Volume 20, Issue 1 Volume 20, Supplement 1 Volume 19, Issue 3 Volume 19, Issue 2 Volume 19, Issue 1 Volume 18, Issue 3 Volume 18, Supplement 1 Volume 18, Issue 2 Volume 18, Issue 1 Volume 17, Issue 3 Volume 17, Issue 2 Volume 17, Issue 1 Volume 16, Issue 3 Volume 16, Issue 2 Volume 16, Issue 1 Volume 1, Supplement 1 Volume 15, Issue 3 Volume 15, Issue 2 Volume 15, Issue 1 Volume 14, Issue 3 Volume 14, Issue 2 Volume 14, Issue 1 Volume 13, Issue 3 Volume 13, Issue 2 Volume 13, Supplement 1 Volume 13, Issue 1 Volume 12, Issue 3 Volume 12, Issue 2 Volume 12, Issue 1 Volume 11, Issue 3 Volume 11, Issue 2 Volume 11, Supplement 1 Volume 11, Issue 1 Volume 10, Issue 3 Volume 10, Issue 2 Volume 10, Issue 1 Volume 9, Issue 3 Volume 9, Issue 2 Volume 9, Issue 1 Volume 8, Issue 3 Volume 8, Issue 2 Volume 8, Issue 1 Volume 6, Issue 3 Volume 7, Issue 1 Volume 7, Issue 2 Volume 7, Issue 3 Volume 4, Issue 3 Volume 5, Issue 1 Volume 5, Issue 2 Volume 5, Issue 3 Volume 6, Issue 1 Volume 6, Issue 2 Volume 3, Issue 1 Volume 3, Issue 2 Volume 3, Issue 3 Volume 4, Issue 1 Volume 4, Issue 2 Volume 1, Issue 2 Volume 2, Issue 1 Volume 2, Issue 2 Volume 2, Issue 3 Volume 1, Issue 1