Objective: The objective of this study is to describe the potato dextrose agar (PDA) with rose-Bengal and chloramphenicol as a new and simple medium (R-PDA chloramphenicol agar) to facilitate the detection of black yeast, Exophiala dermatitidis compared to ready-made conventional medium, namely rose-Bengal chloramphenicol agar.
Methods: We prepared a new medium by adding chloramphenicol and rose-Bengal to ready-made PDA.
Results: This medium proves better growth of the black yeast in terms of increased colony size compared to commercial rose-Bengal chloramphenicol agar. The increase in colony size aids for distinguishing the slow growing black yeast from all the other filamentous fungi.
Conclusions: Compared to traditional rose-Bengal chloramphenicol agar, R-PDA chloramphenicol agar is superior to isolate Exophiala dermatitidis among other fast growing filamentous fungi which are present in the environment samples.
Cite this article as: Jayaram M, Nagao H. Potato dextrose agar with rose-Bengal and chloramphenicol: A new culture medium to isolate pathogenic Exophiala dermatitidis from the environment. Klimik Derg. 2018; 31(1): 11-5.