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Volume 24, Issue 3

Original Article

Entamoeba histolytica Lectin Antigen from Stool Specimens by ELISA Method: 3-Year Data

Pelin Yüksel
Affiliations
  • İstanbul Üniversitesi, Cerrahpaşa Tıp Fakültesi, Mikrobiyoloji ve Klinik Mikrobiyoloji Anabilim Dalı, İstanbul, Türkiye
,
Deniz Gözde Çelik
Affiliations
  • İstanbul Üniversitesi, Cerrahpaşa Tıp Fakültesi, Mikrobiyoloji ve Klinik Mikrobiyoloji Anabilim Dalı, İstanbul, Türkiye
,
Zeynep Güngördü
Affiliations
  • İstanbul Üniversitesi, Cerrahpaşa Tıp Fakültesi, Mikrobiyoloji ve Klinik Mikrobiyoloji Anabilim Dalı, İstanbul, Türkiye
,
Tevhide Ziver
Affiliations
  • İstanbul Üniversitesi, Cerrahpaşa Tıp Fakültesi, Mikrobiyoloji ve Klinik Mikrobiyoloji Anabilim Dalı, İstanbul, Türkiye
,
Sena İzmirli
Affiliations
  • İstanbul Üniversitesi, Cerrahpaşa Tıp Fakültesi, Mikrobiyoloji ve Klinik Mikrobiyoloji Anabilim Dalı, İstanbul, Türkiye
,
Hakan Yakar
Affiliations
  • İstanbul Üniversitesi, Cerrahpaşa Tıp Fakültesi, Mikrobiyoloji ve Klinik Mikrobiyoloji Anabilim Dalı, İstanbul, Türkiye
,
Suat Sarıbaş
Affiliations
  • İstanbul Üniversitesi, Cerrahpaşa Tıp Fakültesi, Mikrobiyoloji ve Klinik Mikrobiyoloji Anabilim Dalı, İstanbul, Türkiye
,
Mustafa Aslan
Affiliations
  • İstanbul Üniversitesi, Cerrahpaşa Tıp Fakültesi, Mikrobiyoloji ve Klinik Mikrobiyoloji Anabilim Dalı, İstanbul, Türkiye
,
Bekir Kocazeybek
Affiliations
  • İstanbul Üniversitesi, Cerrahpaşa Tıp Fakültesi, Mikrobiyoloji ve Klinik Mikrobiyoloji Anabilim Dalı, İstanbul, Türkiye
  • ELİSA
  • Entamoeba histolytica
  • lectin antigen

Abstract

Objective: In this study, we aimed to retrospectively evaluate the distribution of antigen positivity and its relation with demographic data in stool samples from patients with clinical gastroenteritis findings sent to our faculty Serology-ELISA Laboratory between January 2007 and December 2009 for the amoeba antigen test. 

Methods: We investigated 260 kd Entamoeba histolytica Gal- or GalNAc-spesific lectin antigen in stool samples that were sent to Serology/ELISA Laboratory from different clinics and outpatient clinics using ELISA (Ridascreen Entamoeba [c1701], R-Biopharm, Germany).

Results: 33 (7%) of 476 stool samples that were admitted for antigen test were determined as positive. Of 33 cases, 11 (33%) were between the ages of 41-50. Amoeba antigen positivity was detected mostly in autumn months in 14 (42%) cases.

Conclusions: Because of the low sensitivity of direct microscopy, we suggest that antigen detection using ELISA is beneficial for confirming the diagnosis in patients suspected of having amebiasis, to determine the treatment or to prevent unnecessary treatment.

  • 150-153
  • 10.5152/kd.2011.37
  • July 23, 2019
  • 2138
  • 662
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  • This work is licensed under a Creative Commons Attribution-Non Commercial 4.0 International License. CC BY-NC
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