ISSN 1301-143X | E-ISSN 1309-1484
Original Article
Potato Dextrose Agar With Rose-Bengal and Chloramphenicol: A New Culture Medium to Isolate Pathogenic Exophiala dermatitidis From the Environment
1 School of Biological Sciences, Universiti Sains Malaysia, Minden, Pulau Pinang, Malaysia  
Klimik Dergisi 2018; 31: 11-15
DOI: 10.5152/kd.2018.05
Key Words: Black yeast, environment, Exophiala dermatitidis, pigeon, rose-Bengal


Objective: The objective of this study is to describe the potato dextrose agar (PDA) with rose-Bengal and chloramphenicol as a new and simple medium (R-PDA chloramphenicol agar) to facilitate the detection of black yeast, Exophiala dermatitidis compared to ready-made conventional medium, namely rose-Bengal chloramphenicol agar.


Methods: We prepared a new medium by adding chloramphenicol and rose-Bengal to ready-made PDA.


Results: This medium proves better growth of the black yeast in terms of increased colony size compared to commercial rose-Bengal chloramphenicol agar. The increase in colony size aids for distinguishing the slow growing black yeast from all the other filamentous fungi.


Conclusions: Compared to traditional rose-Bengal chloramphenicol agar, R-PDA chloramphenicol agar is superior to isolate Exophiala dermatitidis among other fast growing filamentous fungi which are present in the environment samples.


Cite this article as: Jayaram M, Nagao H. Potato dextrose agar with rose-Bengal and chloramphenicol: A new culture medium to isolate pathogenic Exophiala dermatitidis from the environment. Klimik Derg. 2018; 31(1): 11-5.

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